Ultrasonography interconverts electric and acoustic energy to create a gray-scale image of internal anatomy for diagnostic procedures . Since the early 1980s ultrasonography has been conducted on a variety of important fish species such as salmon, catfish, sturgeon, and bass to identify sex and to evaluate gonadal development. Understanding the reproductive cycle is crucial for management of broodstock and increased control of fish reproduction. Female Nile T ilapia are asynchronous spawners ( follicles in all stages of development present in ovary). This hinders broodstock from spawning at the same time . Non-invasive methods for assessing and monitoring tilapia reproductive biology are limited. Ultrasonography provides a real-time and safe method for monitoring, evaluating and segregating broodstock females based on ovarian development. S ystematic fish handling and ultrasound imaging procedures and interpretation guide based on the ovarian cycle for Nile Tilapia was developed at Kentucky State University . This will be used to develop educational material s to provide training to hatcheries for improving broodstock management, spawning synchronization, and hatchery productivity (Figure 1). F emale broodstock c an be grouped and separated into a scheme of tanks based on ultrasound imaging and the ovarian cycle . This will be instrumental for ease of egg production that is predictable, reduces fish handling and labor, and uses time and space affectively for increasing reproductive efficiency and hatchery productivity .