For almost five decades, the unilateral eyestalk ablation (hereafter named ablation) has been used to accelerate the gonadal maturation of broodstock females from several species of penaeid shrimp (e.g., Penaeus, Litopenaeus and Farfantepenaeus species). And why not, if the ablation results in regular peaks of maturity and spawning, making it easier to plan production schedules in hatchery facilities. However, there is growing public and animal welfare concern about this procedure, which may result in its prohibition in the future. As a result, there is a need to induce a transition and paradigm shift regarding the employment of this technique. Despite efforts in the literature dating back to the 1980s to leave the shrimp to mature naturally, the findings were contradictory. However, broodstock management has evolved over time, and significant strides in broodstock output have been made (e.g. diet, pre-maturation culture systems and maturation management). As a result, there is a rising interest in investigating how ablation and broodstock origin - alternative culture procedures - affect reproductive performance and offspring quality. Over the last five years, on a series of experiments, our laboratory has investigated the effects of ablation and the biofloc system ( vs clear-water) on a native penaeid shrimp species, F. brasiliensis , and an exotic species, L. vannamei . According to our findings, ablation increased female mortality in both species. When both species came from clear-water, the quantity of eggs produced by ablated and non-ablated females was comparable, however evidence suggests that L. vannamei produced more eggs when it was collected from biofloc. Furthermore, there was substantial evidence that non-ablated females generated higher quality offspring, and this was especially true for eggs produced by L. vannamei from biofloc origin. All these findings suggest that both ablation and origin may affect the reproductive performance and offspring quality of penaeid shrimp species, and that the lower number of spawning and predictability from non-ablated females can be remedied by increasing the number of reproductive females, which can live longer and produce higher-quality offspring.