Live food is an essential part of the nutrition of the larval stages of different aquaculture species. Its quality is closely related to the means of fertilization used for its cultivation, being the F/2 Guillard the most used. Chemical silage from fish waste, with high nitrogen and carbon content, can be an alternative. In this study, the effect of chemical silage from fish waste as a supplement, complement and total substitute for the commercial culture medium F/2 Guillard on the growth of the microalgae Tetraselmis
chuii and Chaetoceros
muelleri was evaluated.
The silage was made with waste from the “Chac-chi” fish (Haemulon
plumierii ) according to Gallardo et al (2012) from the catch of riverside fishermen from the port of Sisal, Yucatan, Mexico. The pH and soluble protein content during silage was recorded (31 days).
Two experiments were carried out to evaluate the effect of partial and total replacement of the F/2 Guillard medium on the population growth of T. chuii (Exp. 1) and Ch. muelleri (Exp. 2) (Table 1) during 10 and 8 days respectively. The cultures of both microalgae were carried out in 500 ml Erlenmeyer flasks.
Silage as a complement and supplement to F/2 Guillard allowed higher concentrations (cell ml-1 ) of T. chuii with TB and TA on day 9 of culture, respectively (Fig. 1a). In the case of diatoms, the highest concentration was obtained on day 5 of culture with the TA (5.15 x 10-6 cel ml-1 ) and where only the silage (TC) was used (4.4 x 10-6 cel ml- 1) (Fig. 1b).
The results obtained indicate that the chemical silage of fish waste with a high content of nitrogen and other elements, provided nutrients that were beneficial for the growth of microalgae and that it can be used as an enrichment or partial replacement of the F/2 Guillard medium for T. chuii . In the case of Ch. muelleri , the results indicate that the silage allowed a total replacement of the commercial medium F/2 Guillard.
Literature
Gallardo P., …., and Sotelo A. 2012. Nutritive value of diets containing fish silage for juvenile Litopenaeus
vannamei (Bonne, 1931) . Journal of the Science of Food and Agriculture (DOI 10.1002/jsfa.5632). 2320 -2325
Acknowledgments