The present study tested the effects of environmental factors in total arsenic accumulation by Sarcodia suiae. The alga, S. suiae was exposed individually to a range of temperature (15, 20, and 25 °C), and LI (30, 55, and 80 μmol photons m−2 s−1) at initial arsenate [As(V)] concentration (iconc: 0, 62.5, 125, 250, and 500 μg L−1) conditions. Temperature significantly affected TAs production and maximum absorption were obtained at 15 °C, which was significantly stimulated by increasing iconc. On the other hand, LI had no significant effect on TAs production, although maximum absorption was estimated in 80 μmol photons m−2 s−1. Stronger effects in morphology were observed in higher iconc although the factor (temperature or LI) did affect the difference somewhat. The uptake results depended upon environmental factors (temperature, LI, and iconc) that may provide important insights regarding particular culture conditions for efficient wastewater treatment processes for selective algal species. Yet, future studies are needed to further the understanding of As metabolic processes by this alga to ascertain their effectiveness if used for bioremediation.