New technologies have been introduced by the ethanol industry to improve the efficiency of ethanol production, resulting in new types of distiller dried grain with different nutrient profiles. One of the new processing techniques removes fibrous corn components before fermentation and removing the soluble fraction after fermentation to produce a range of high protein distiller grain with yeast which contain from 40% (HP40Y) to 49% (HP50Y) protein. The current study was conducted to evaluate the efficacy of HP50Y and HP40Y as a replacement for corn protein concentrate (CPC) in diets of pacific white shrimps, Litopenaeus vannamei. In the growth trial, graded levels of HP50Y (0.0, 5.0, 10.0, 15.0 and 20.0%) were used to replace CPC (13.1, 10.0, 6.6, 3.5 and 0.2%) In the second series of diet, graded levels of HP40Y (5.0, 10.0, 15.0 and 20.0%) were used to replace CPC (10.5, 8.0, 5.5 and 2.5%) which was evaluated over a 40 days growth trial (initial weight 0.54 ± 0.01 g; n=4). At the conclusion, no significant differences were detected in growth, FCR, survival, food consumption and net protein retention of shrimp (P-value>0.05). However, results from regression analysis revealed that there was a significant increase (p-value=0.04; r2= 0.20) (p-value=0.02; r2= 0.25) in weight gain percentage of shrimp as the percentage inclusion level of HP50Y and HP40Y have increased in the diets of shrimp. Regarding net protein retention (NPR) there was no significant differences (P-value>0.05) among all treatments but from the linear regression analysis it came to know that NPR was going to be increase and there was a significant increase as the inclusion levels of HP50Y (p-value= 0.05; r2= 0.19) and HP40Y (p-value= 0.03; r2= 0.23) increased in the diets. The proximate whole-body composition of shrimp was indicated that there were no significant (P>0.05) differences between moisture, protein, fat, and ash of shrimp body. In summary, it was indicated that HP50Y and HP40Y both are good protein source and can be used up to 20% inclusion level in the diets of shrimp. In addition, the HP40Y and HP50Y also contains an elevated level of yeast, stimulating growth and could possibly enhance immune response of shrimp. Further studies should contemplate the supplementation of HP40Y and HP50Y as an effective protein source for the diets of carnivorous aquaculture fish species, for instance trout and other salmonids.