ELECTRIC SHOCK EFFECTIVENESS ON THE VIABILITY OF MUSTIKA CARP (Cyprinus carpio) SPERM CELLS  

Mustika is superior strain carp that formed by Research Institute for Fish Breeding is a superior breeding fish breeding by selective breeding program based on the molecular mark of MHC II with purposing to increase KHV disease resistant. Furthermore, increasing of Mustika carp genetic quality can be did by introduction of gene with electrophorese method. Electrophorese will be done by inserting gene transgene to gene target which later be expressed to increasing more genetic quality of Mustika carp. Gene inserted can be done by give electric shock though electrophorator machine to sperm cells so that making ease for transgene gene to inserting genetically structure of target gene. The aim of this research is to knowing the effectiveness of electric shock to viability of Mustika carp sperm cells.

This research used experimental method with three times repetitions on each treatment. 1 mL a total of Mustika carp sperm cells diluted to physiological solution (NaCl) with 10 times of dilutions, then electric shock given by Gene Pulser Xcell Bio-Rad electrophorator machine on 0, 25, 50, 75, and 100 volt with 0.5 mS long exposure and 0.1 S of resting time at three and five times. Viability of sperm cells observation used eosin 0.01g/mL coloration method, observed by under microscope with 400x magnification, and count based on the percentage of life sperm cells/total sperm cells.

Based on the observation, we obtaining the number of sperm viability at three times electric shocked on 0, 25, 50, 75, and 100 volt are 100, 90, 88, 80, and 70%; while at five times electric shocked on 0, 25, 50, 75, and 100 volt are 100, 88, 82, 75, and 57%. This show that power and number increasing of electric shock can be following by decreasing of sperm cells viabilities.