Differentiation of Two Coregonidae Species by the RAPD-PCR Method
At present time a molecular technique based on the polymerase chain reaction provides an effective tool for the rapid identification of genetic markers. We used polymerase chain reaction with random primers (RAPD-PCR) to examine interspecific variations in two species of Coregonidae Coregonus muksun and Coregonus peled. We screened a set of 10-bases primers and chose six of them that gave discreet bands assumed to be monomorphic characters. High significance and reproducibility of amplified DNA spectra for each of species were demonstrated. We found out that RAPD-spectra for Coregonus muksun and Coregonus peled contained a number of bands common for both species. However analysis of RAPD-patterns revealed several fragments that could be considered as species-specific markers for differentiation of Coregonus muksun and Coregonus peled.