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Add To Calendar 27/04/2016 14:10:0027/04/2016 14:30:00America/ChicagoAsian-Pacific Aquaculture 2016RAPID DETECTION FOR IDENTIFICATION SOME BACTERIA DISEASE IN FISH1) Crystal 4The World Aquaculture Societyjohnc@was.orgfalseanrl65yqlzh3g1q0dme13067DD/MM/YYYY


E.B. Kholidin2), Indri A3), Padel P. 3), Kusnur H3)., Arif R.E.4), Messy S4), Zulkarnain4)
1) Paper presented at the Asian Pacific Aquaculture, 26-29 April 2016, Grand City, Surabaya
2) Engineer at Jambi Freshwater Aquaculture Center (JFAC)
3) Assistant Engineer at Jambi Freshwater Aquaculture Center (JFAC)
Jl. Lingkar Selatan RT 24, Kel. Paal Merah, Kec. Jambi Selatan - Kota Jambi



Recently, bacteria disease detection and identification are using biochemical examination such as Gram Staining, Ryu, motility, TSIA, Indol, MrVp, Glucose, Sucrosa, Lactose, Manitol, Inositol etc. It takes time and need many conditions to get accurate results and also sometime the result is still debatable.

In 2015, we develop one method to detection and identification some bacteria disease. It will take time only around 24 hour for us to determine the bacteria disease.  This method known as serum antibody.  With this method, we can determine and identification a disease caused by bacteria quickly and accurately. By this method, farmer lost's caused by high mortality will reduce.

Each individu has specific antibody that only can react between them.  This antibody will not react with another individu.  So, we develop specific  antibody from some bacteria (6 bacteria) such as Edwardsiella ictaluri, Edwardsiella tarda, Streptococcus iniae, Aeromonas salmonicida, Aeromonas hydrophyla and Pseudomonas anguilliseptica.

First, we use original bacteria come from ATCC (American Type Culture Collection) as source of each bacteria.  After each bacterium was grown and tested biochemistry, each bacteria were mass culture on TSA media for 24 hours.  Bacteria were harvest use NaCl  fisiologis and scrub with Ose needle.  Next step is make antigen O and antigen H.  Non motile bacteria are usually can make only antigen O and motile bacteria can make antigen O and antigen H.

Finally, we use rabbit as a recipient and inject using a syringe.  We inject rabbit for 4 weeks (one week one injection following doses  0, 5 ml; 1 ml; 2 ml; 3 ml).  One day before fifth week, rabbit antibody check by using 0, 2 ml of antibody taken from rabbit, if titer antibody shows high titer (640), antibody ready to harvest. If less than 640, inject again 3 ml antigen O or antigen H to rabbits.  

Serum antibodies were stored at temperature 4 °C and ready to use for the rapid identification of fish disease caused by bacteria.

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