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Add To Calendar 29/04/2016 14:50:0029/04/2016 15:10:00America/ChicagoAsian-Pacific Aquaculture 2016Lysine Supplementation in Diets with Fermented Rubber Seed Hevea Brasiliensis Meal For Nile Tilapia  Oreochromis niloticus. VIP Room 1The World Aquaculture Societyjohnc@was.orgfalseanrl65yqlzh3g1q0dme13067DD/MM/YYYY

Lysine Supplementation in Diets with Fermented Rubber Seed Hevea Brasiliensis Meal For Nile Tilapia  Oreochromis niloticus.

Muhammad Agus Suprayudi,  Didi Humaidi Yusuf, Dedi Jusadi, Julie Ekasari, M zairin Junior.
Depaert Of Aquaculture
Bogor Agricultural University
Jln Lingkar Kampus IPB Darmaga, Bogor 16680

This research was conducted to evaluate lysine supplementation in RSMF as protein source in nile tilapia diets.The fish with an initial body weight 6,29 ± 0,12 g were reared for 40 days in indoor tanks (100x50x50 cm) and fed three times daily to satiation level, at 08.00 am, 12.00 pm and 16.00 pm. This research consisted of five treatments in triplicates. The differences between treatments were the protein contribution of RSMF in replacing soybean meal as the protein source with and without lysine supplementation in the diets as follow: 0% protein of RSMF as control;50% RSMF; 50% RSMF supplemented with 0,15% of lysine; 75% RSMF and 75% RSMF with 0,3% lysine supplementation. Growth performance, amino acid retention,  blood chemical, hepatosomatic index (HSI),  survival, blood parameters (erythrocite, leucocyte, hematocrit and haemoglobin) and cyanide content (test diets and whole body) was used as the observed parameters.  

The results showed that lysine supplementation at 50% and 75% RSMF did not signifcantly affected cholesterol, LDL, HDL and plasma protein (Table 1). Lysine supplementation could increase amino acids and protein retention. Protein retention of 50% and 75 % RSMF without lysine supplementation were 30,28% and 21,10%, respectively and were increased after lysine supplementation at 50% and 75% RSMF up to 35,34% and 27,19% (Table 2). The increase of protein retention was followed by the increase of feed efficiency, final body weight and daily growth rate. The blood parameters showed that erythrocytes, leucocytes and hematocrit were still in normal levels except hemoglobin which was rather low possibly due to stress caused by cyanide. HCN content in fish whole body after the rearing period was still within acceptable range. This study clearly indicates that quality of RMSF could be improved after lysine supplementation and thus could increase the utilisation of RMSF to replace soybean meal up to 75% as the protein source in nile tilapia diets.

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