Field Test Application of Bivalent Vaccine to Prevent Motile Aeromonas Septicemia and Mycobacteriosis Disease on Giant Gourami

Desy Sugiani*, Otong Zaenal Arifin, Tatik Mufidah, and Uni Purwaningsih
 
Research Institute for Freshwater Aquaculture
Jl. Sempur No. 1 Bogor - West Java, INDONESIA
desysugiani@yahoo.co.id  

Vaccination of fish was recommended for use nationwide in all fish farming centers mainly in Minapolitan fish aquaculture to support industrialization and to achieve optimization targets of fish vaccination movement in Indonesia. Coinfection diseases of Motile Aeromonas Septicemia (MAS) and Mycobacteriosis is the infectious bacterial disease that cause hemorrhagic septicemia in Giant Gourami (Osphronemus goramy) and it can lead fish mortality 30-80%.

Different vaccine preparations and formulations for vaccination were tried by adding formalin to the bacterial culture (bacterin) of Aeromonas hydrophila and Mycobacterium fortuitum and were made as the bivalent vaccine. There are several factors that needed to be considered when using vaccines such as safety level, sterility of the product, vaccine composition, and the effect of yield gap that will differentiate protection results at the application with limited scale and in the field test application.

Survival rate of Giant Gourami at the field test application of bivalent vaccine was 51.4% while the controls without vaccination was 26.67%. The bivalent vaccine can improve survival rate of fish 24.73% higher compared with the group of fishes without vaccinated. Details of survival rate of fish were vaccinated and unvaccinated can be seen in Table 1. Locations of the fish pond were separated among several locations with different environmental characteristics based on topography elevation above mean sea level/AMSL. Details of the results of measurements of some water quality parameters can be seen in Table 2.