THE SELECTION AND CHARACTERIZATION PROBIOTIC CANDIDATE FOR CONTROL NITROGEN COMPOUNDS IN FRESHWATER AQUACULTURE

Waste of intensive aquaculture containing high nitrogen compounds have resulted decreasing of the water quality. Therefore, an additional of probiotics as bioremediationto improve water quality is needed The aims  of this experiment was to obtain nitrification and denitrification bacteria as a potential probiotic candidates to control nitrogen compounds in the pond water.

This experimen consisted of two steps, namely: 1) the collection of bacteria from water and sediments on the pond catfish in the Pudak Muaro Jambi and the Koto Mesjid Kampar, Riau; 2) testing of candidate probiotic bacteria in vitro consisting were as followed: 1) Isolation and selection of bacterial nitrification and denitrification; 2) morphological characterization of bacterial nitrification and denitrification; 3) Characterization of physiological / biochemical nitrification and denitrification bacteria selected; 4) Characterization of genetic nitrification and denitrification bacteria selected with 16S- rRNA sequencing. Descriptive data analysis was performed.

The results showed that there were fourth isolates bacteria nitrification namely NP1; NP2; NP3; NP4 and fourth isolates denitrification namely DP1; DP2; DP3; DP4 which had potential as a candidate probiotic in term of  controlling nitrogen compounds (Tabel 1, 2) The nitrification isolates  could reduce  ammonium (99,37 - 99,87%), nitrite accumulated (0,05 - 0,06 %) and nitrate accumulated (1,56 - 2,08 %). While the denitrification isolates could reduce  nitrate (99,9 -100%) and nitrite accumulated  (0,00 - 0,04 %)(Tabel 3,4)