Disinfectant APPLICATIONS in Barramundi (Lates calcarifer, Bloch) Hachery (Larviculture)

 
Agung Darmono*, Antin Sri Lestari and Joni Agus R.
 
Batam Mariculture Development Centre
Jalan Raya Barelang, Pulau Setoko, Jembatan III
PO BOX 60 Sekupang, Batam 29422
Email : darmonoseribu3@gmail.com  

One of the factors that influence the success of hatchery are environmental factors in Barramundi hatchery . Water as a medium of live fish often fluctuate, either daily, seasonal, and certain extreme conditions. Fluctuations in the water  factors affect to the organisms, especially aquatic organisms. Bacteria is one of the organisms in the waters that has a function that can be beneficial or harmful for marine aquaculture activities. The adverse effects of these bacteria need to be reduced or even eliminated by reducing the population of harmful bacteria. One way to reduce the population of bacteria that were in the water with the use of compounds in plants such as saponins. So with saponin as an antimicrobial can function as a disinfectant in the water. It  expected to decrease in the population of bacteria in the water and can improve water quality.

This activity begins with preparing the larval rearing tubs with a volume of 12 m3. Barramundi larvae (Day 1) as much as 20 pcs / liter or 200,000 pcs/tub. The activity  is carried out for 30 days. During the activity would be done feeding, remove waste, water exchange, provision of disinfectant and water quality control. Before the actifity begin, Barramundi larvae would be tested for resistant of disinfectants. The trial  conducted using 5 ppm for 24 hours of immersion in a container volume of 10 liters. Indicators resistance seen after 24 hours the larvae are still alive. Bacterial test against larvae media is done by counting the number of TBU and TBV in the media before the immersion activity  with disinfectant  every 2 days. The treatment given is the control, of 2.5 ppm and 5 ppm for 10 days of maintenance. Bacteria Test against larva is done by checking the larvae after soaking in disinfectant. Visual observation was doing every day. Counting the number of fish that live performed at the beginning and end of activity. Bacteria test for water and larvae every 2 days.

Preliminary trials larvae resistance to disinfectants performed using a dosage of 5 ppm for 24 hours by immersion 200 larvae in the water volume of 10 liters, which results in improved survival rate of larvae by 93%. Results of testing the effects of disinfectants on bacteria populations in larval rearing showed that immersion disinfectant dose of 5 ppm obtained bacterial population is lower than immersion disinfectant at a dose of 2.5 ppm and without disinfectant. Results of checks bacteria on barramundi larvae showed that the immersion disinfectant dose of 5 ppm and 2.5 ppm showed a smaller value than without immersion (control).