EVALUATION OF BACTERIA CONSORSIUM ON INCREASING QUALITY OF PLANT FEED INGREDIENTS

Availability of plant of Indonesia was abundance and varies in species at which probably potential for feed ingredient candidates, particularly as carbohydrate source. However, the weakness of using plant material is that they contain high fiber, difficulty to be digested by fish. In nature, garbage or litter of plant materials was processed by microbe to reduce its size to avoid its accumulation. Herbivorous fish species could be digested plant diet using various cellulolytic microbes attaching in the diet. If plant ingredients are reduced their crude fiber using cellulolytic microbes, it probably will improve their quality. Many bacteria producing cellulolytic enzyme to degrade crude fiber, but a few is able to produce completely to synthesis enzyme system and hydrolyzing crystalline material. This study therefore proposes to evaluate bacteria consortiums for collecting cellulose enzyme completely for degrading plant ingredients more effectively.

There were three steps in this study, namely (1) selection of isolate cellulolytic bacteria collected at 2012 (UG3, UG5, UG7) and 2013 (TS2b, SS4b1), respectively as a candidate of microbe consortium using parameters of total cellulose activity (filter paperase), endoglukanase (CMCase) and exoglukanase (avicellase); (2) characterization of microbe consortium (antagonistic test and enzyme cellulose activity in term of specific and total; and (3) test of their effectiveness utilization for improving quality of plant ingredients (Rhodimenia sp) using treatments of various levels of cellulotic bacteria of 10¹² cfu per mL in concentration at 0, 3, 6 and 9%, respectively. The results showed that there four isolates having the highest values of total cellulose activity, endoglukonase and exoglukonase, namely UG5, UG8, TS2b and UG7. Isolate of TS2b had antagonistic character to UG5 and UG7 when they were cultured together. Total activity cellulose and endoglukonase of consortium between UG5 and UG8 was lower than at consortium, but these values were high at consortium of UG5, UG8 and UG7 compared at individual culture. Fermentation of Rhodimenia sp using the consortium increased sugar reduction level as well as diluted protein.