THE ISOLATE ORIGIN OF WSSV DETERMINES THE VIRAL VIRULENCE TO PISANG SHRIMP (Penaeus SP.) AND TIGER SHRIMP (Penaeus Monodon)

Nurbariah, Zahria Zurrah, Royani, Ibnu Sahidhir
 
 Brackishwater Aquaculture Development Center of
Ujung Batee, Aceh, Indonesia
 n03r_97@yahoo.com

Pisang shrimp (Penaeus sp) was founded in Aceh west coast. Usually the culture of pisang shrimp was done together with Tiger shrimp (Penaeus monodon). Meanwhile White Spot Syndrome Virus (WSSV) often affect shrimp culture production. Therefore, this experiment was conducted to know endurance of pisang shrimp against WSSV.

The experiment using intra muscular (im) injection of 0,1 ml isolate to shrimp test. The experiment consists of  two activity i.e activity (1) was challange test by injection pisang shrimp using two different isolate (20 mg/ml) i.e pisang shrimp isolate from Aceh west coast and tiger shrimp isolate from Aceh east coast. Activity (2) was challenge test by injection isolate of tiger shrimp with different consentration (from pure isolate 20 mg/ml was diluted to four serial dilution of 1:10) to pisang shrimp and tiger shrimp group.

The result of activity (1) showed that WSSV infected pisang shrimp faster when injection by pisang shrimp isolate than tiger shrimp isolate. The first mortality occured on pisang shrimp at ±25 hours post injection of pisang shrimp isolate and ±32 hours post injection of tiger shrimp isolate. Histopathology data showed that the number of infected cells post injection by pisang shrimp isolate were more than injection by tiger shrimp isolate (Table 1). The result of activity (2) showed that during 12 days of observation, mortality occured on tiger shrimp group at all of treatment, meanwhile on pisang shrimp group the survival rate only found in treatment of 1:10(3) (25%) and 1:10(4) (20%) (Figure 1). The result indicate that pisang shrimp can be infected by WSSV but it appears that the endurance against WSSV is rely on the origin of the isolate.