World Aquaculture Society Meetings

facebook

Your browser does not support the most current secure communications protocol. The World Aquaculture Society is committed to the security of your private information. In order to accept credit card data on this site we are recquired to be in compliance with Payment Card Industry (PCI) standards. Current PCI standards will not allow us to accept traffic from browsers that do not support TLS 1.2 after June 30, 2018. We are alerting you to the important need to update your browser. Changes to our web server made on or before June 30, 2018 will make was.org unavailable with the browser you are currently using. [More..]

Add To Calendar 27/04/2016 16:00:0027/04/2016 16:20:00America/Los_AngelesAsian-Pacific Aquaculture 2016IMMUNE ENHANCEMENT AND UP-REGULATING IMMUNE-RELATED GENES EXPRESSION IN VANNAMEI SHRIMP Litopenaeus vannamei BY CRUDE FUCOIDAN ISOLATED FROM TROPICAL BROWN ALGAE   VIP Room 2The World Aquaculture Societyjohnc@was.orgfalseanrl65yqlzh3g1q0dme13067DD/MM/YYYY

IMMUNE ENHANCEMENT AND UP-REGULATING IMMUNE-RELATED GENES EXPRESSION IN VANNAMEI SHRIMP Litopenaeus vannamei BY CRUDE FUCOIDAN ISOLATED FROM TROPICAL BROWN ALGAE  

Agus Setyawan*, Alim Isnansetyo,
Murwantoko, Sudarmanto Indarjulianto,
Ch. Retno Handayani
 
Department of Fisheries, Faculty of Agriculture, Gadjah Mada University, Indonesia
*agusu.san@gmail.com

Little study on fucoiodan from tropical brown algae and its bioactivity as immunostimulant in shrimp is found up to now. The purpose of this research were to know the innate immune response and immune-related genes expression of white shrimp, L.vannamei, that was administrated by crude fucoidan from tropical brown algae.

Crude fucoidan was isolated from three brown algae i.e. Sargassum sp., Padina sp., and Turbinaria sp. that were collected from Gunung Kidul coasts, Yogyakarta. The fucoidan was characterized by thin layer crhomatography (TLC) and FT-IR. Fucoidan was administered orally by supplementing in vannamei feed at dosages of 2,5 and 5 g kg-1 feed of each brown algae. Response immune parameters including total hemocyte count (THC), phagocytic activity (PA), phagocytic index (PI), phenol oxidase (PO) activity, superoxide dismutase (SOD), and total protein plasma (TPP) was evaluated at 0, 4, 8, and 12 day post treatment (DAT). Immune-related genes expression include lectin, Toll, LGBP, and proPO was performed by real time qPCR from the selected shrimp haemolymph based on response immune parameters. The β-actin gene was used as an internal control (IC).

Results showed that crude fucoidan isolated from Sargassum sp. overally enhanced non-specific immune resoponse of shrimp vannamei better than Padina sp and Turbinaria sp. Significat increase in non-specific immune response of vannamei shrimp was observed at 4 DAT for THC and PA parameters. Whereas, SOD and TPP increased significantly at 8 DAT. PO activity was observed later at  12 DAT. There was no significant increase in IP after fucoidan treatment. The CT analysis showed that immune-related genes were up-regulated in the range of 2 to 20 fold comparing to IC. The Toll gene of shrimp administered with fucoidan Sargassum sp. was highest upregulated. This research suggest that fucoidan from tropical Sargassum sp. have big opportunity to be developed as micronutrient supplement in shrimp feed to enhance immune response.




Copyright © 2001-2018 World Aquaculture Society All Rights Reserved.