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PERFORMANCE OF F3 TRANSGENIC AFRICAN CATFISH CONTAINING GROWTH HORMONE GENE OF STRIPPED CATFISH (PccBA-PhGH)

Huria Marnis, Selny Febrida, Bambang Iswanto, Imron, Raden Roro Sri Pudji Sinarni Dewi, Sultan Akbar, Fery Jeksen Sihotang.
 
Research Institute for Fish Breeding
marnis.huria@gmail.com
 

Transgenesis can improve the genetic traits of commercially important fishes for aquaculture. Growth performance of fish is influenced by growth hormone and IGF-I hormone. The precise hormonal controlling increased growth rate and associated feed conversion ratio in transgenic African catfish are not yet known. Research Institute for Fish Breeding has produced fast-growing transgenic African catfish by transferring growth hormone gene of stripped catfish into germs cell via electroporation in 2011. The aim of this study was to evaluate the performance of F3 transgenic African catfish.

F3 transgenic has been produced by mating F2 transgenics with non-transgenic. The parameters such as body weight, Feed Conversion Ratio (FCR), growth hormone and IGF-1 hormone profile were used to evaluate the performance of F3 transgenic. Analysis of growth hormone and Insuline-like Growth Factor (IGF-I) hormone was conducted by  Enzyme Linked Immunosorbent Assay (ELISA) method using serum samples.

The performance of F3 transgenic African catfish was significantly better than the non-transgenic (P<0.05). The body weight of transgenic population (484±255.25 g) was higher than non-transgenic siblings (319.98±165.27 g) after 8 months of rearing (Figure 1). FCR of transgenic (0.89) was lower than non-transgenic (1.30). Growth hormon of transgenic (5,67±2,65 ng/mL) was higher than non-transgenic (3.00±1.41 ng/mL) (Figure 2), in addition, IGF-1 hormone of F3 transgenic (6.63±0.11 ng/mL) was higher than non-transgenic (5.38±0.63 ng/mL) (Figure 3). High level of growth hormone and IGF-I hormone represent both growth performance and efficiency of feed utilization of transgenic African catfish.




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