INFLUENCE OF CARBOHYDRATE ENRICHED DISTILERY EFFLUENT (MF-CEED) ON AEROBIC MICROBIAL FLOCCULENT DRIVEN NURSERY RACEWAYS TECHNOLOGY FOR PACIFIC WHITE SHRIMP, Litopenaeus vannamei.
For the First time in India,To tackle the exisiting challenges in the shrimp farming,an innovative investment has been made to turn the weakness into strength by deploying Carbohydrate Enriched Distillery Effluent(MF-CEED) ,unwanted residual liquid waste as the potential carbon source for the Aerobic microbial Flocculent Technology.
The aim of this study was to evaluate the potential use of Carbohydrate Enriched Distillery Effluent (MF-CEED) in Aquaculture and to study the changes in the water quality in the nursery rearing of Pacific White Shrimp (Litopenaeus vannamei) cultured in Aerobic microbial flocculent system.
For the nursery phase,postlarvae (PL10-12,0.02 ±0.01gm) were stocked at a density of 6000 PL/m3 in two 50 m3 raceways respectively and reared for 21 days.The water was maintained with constant aeration and the temperature was controlled (30 ± 1˚c).Post Biomethanated Distillery Effluent with enriched carbohydrate was used for carbon supplementation to provide 6g of carbon for each 1 g of total ammonia nitrogen (TAN) as measured in the water.Temperature and Dissolved oxygen were measured thrice a day.Shrimps were fed six times a day with a commercial diet containing 40% crude protein.Mean wet weight was sampled once per week to calculate the weight gain and adjust feed.In the first 10 days of the experiment,the nitrogen of the ration was controlled using a carbon/nitrogen ratio of 15:1.MF-CEED was used as organic carbon source (40% carbon) to keep ammonia levels (TAN) upto 1 mg L-1.The selected water quality parameters like Ammonia,nitrite,nitrate,alkalinity,pH,salinity,TSS were monitored daily. At the conclusion of the trial survival was 91.2% and final weight was 0.5g.Shrimp grew at an average of 2.2-2.3 g/week and the FCR was 1.7-1.9.All water quality parameters were within the range suitable for Litopenaeus vannamei culture.Mean temperature,salinity,DO and pH were 29.9 ,6.8 ppt ,6.67 mg/L and 7.9 respectively.Mean ammonia and nitrite were 0.67-0.84 mg/L and 1.30-2.20,respectively.TSS remained below 400 mg/L for the duration of the trial.Green-colony forming vibrio concentrations remained below 10 CFU/mL and less than 2% of the yellow-forming colony concentrations throughout the trial.The study revealed rapid reduction of TAN and Nitrite -Nitrogen and did not result in significant effect on water quality or on shrimp performance.These results demonstrate that MF-CEED can be used as the effective carbon source for the nursery culture of Litopenaeus vannamei.