APPLICATION OF THE MICROSATELLITE MARKERS IN THE SHRIMP BREEDING PROGRAMS

Mohamed E. Megahed; Elmesiry G E; Mervat A.M.A; Amal M.I. El-feky;Hanaa A.H. Fayed; Hoda Eisa

Correspondence: Mohamed E. Megahed, National Institute of Oceanography and Fisheries, Gulfs of Suez & Aqaba's branch, Suez, Egypt.

Microsatellites are ideal genetic marker for applications in aquaculture. Microsatellites have properties which make them very suitable for the study of pedigree analysis in selective breeding programs. Most of the microsatellites are noncoding, and therefore variations are independent of natural selection. This paper addresses the applications of microsatellite markers in the breeding programs for closed thelycum shrimp Fenneropenaeus indicus in the context of management of the breeding programs. This technique was used to construct pedigree tree for the identification of the parents (Males and Females) and their offspring. A total of 100 Microsatellite markers were developed in F.indicus. 10 Microsatellite markers were suitable to determine the allelic inheritance and paternity within the shrimp families. All of the offspring were correctly assigned to their parents, shown to be genetic descendants of their presumed parents and showed Mendelian inheritance. The results of this paper showed that microsatellite markers are useful tool for animal breeders in genetic management of the applied breeding programs.