EVALUATION OF THE ANTIMICROBIAL ACTIVITY OF MUCUS EPIDERMAL NILE TILAPIA Oreochromis niloticus AGAINST BACTERIA OF THE GENUS Vibrio AFFECTING WHITE SHRIMP Litopenaeus vannamei

Máximo García-Marciano^*, Apún-Molina J. P., Sainz-Hernández J. C., Santamaría-Miranda A., Medina-Godoy S., Ruiz-Vergara L. R., Cervantes-Mejía C. I., Gonzalez-Felix G. K.
Instituto Politécnico Nacional-Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional Unidad Sinaloa. Boulevard Juan de Dios Bátiz Paredes # 250, Guasave, Sinaloa México 81101. mgarciam1515@alumno.ipn.mx.

The outbreaks of diseases in shrimp farming, has generated economic great losses, mainly caused by pathogenic organism in the countries Latin American. The alternative for counter this problem in the shrimp farming is the system of polyculture, this system generates at shrimp some benefic by the presence of fishes in the ponds with the shrimp. On the other hand, the presence of small molecules related to the immune response has been confirmed, in the epidermal mucus of the some fish important in aquaculture. This studio try to confirm antimicrobial activity of the extracts obtained from the two tilapias varieties of Tilapias Oreochromis niloticus and Oreochromis sp against pathogenic bacteria of white shrimp Litopenaeus vannamei. The Tilapias Oreochromis niloticus was proportionated from fry production laboratory, while that the other variety was captured in mats (Adapted to the marine environment) connecting to Navachiste Bay, Sinaloa, México.

The epidermal mucus was collected to perform the acidic, organic and aqueous extractions. We assessed the antimicrobial activity of each of the extracts against Vibrio parahaemolyticus strain E9-2 and ATCC 17802, and V. harveyi CAIM 1792 using the turbidimetric method. The percentage of inhibition was assessed with the values obtained. A total of eight extracts (table 1) of the two tilapias varieties were obtained. Each one of the extracts was adjusted to the concentration of 1 mg mL^-1 of dry weight in distilled water. The antibacterial activity of each one of the extracts was different for each of the strains tested. With the tests carried out in the present study, the presence of antimicrobial activity of the different extracts of the epidermal mucus of the two tilapia varieties was confirmed. So it can be concluded that in each of the different extracts are present antimicrobial agents with activity that could be bacteriostatic, bactericide or bacteriolytic.