WASTEWATER AND TEXTILE INDUSTRY EFFLUENT TOXICITY: DECLINING TRENDS IN LIPID PROFILE IN THE GONADS OF Labeo rohita
The study was designed to investigate the comparison of lipid profile in the gonads (ovaries/testes) of Labeo rohita exposed to wastewater and textile industry effluents. Fingerlings of major carps were procured from fish seed hatchery and acclimatized to laboratory conditions for two weeks prior to experiments. After acclimatization fish were divided into three groups one for control and others two as experimental. LC50 for 96 hours was determined for Labeo rohita (150.40 ml/l) in Textile industrial effluent and (227.93 ml/l) in sewage water. The sublethal concentrations were of textile industrial effluent (120, 100 and 75 ml/l) and for sewage water (220, 200 & 175 ml/l), respectively for three months. The morphometric parameters were measured fortnightly. The fingerlings were fed @3-4% body weight. Physico-chemical parameters of water (Temperature, DO, EC, TSS, TDS, and pH) were measured on daily basis. After the completion of experimental estimation of total lipids, cholesterol, phospholipids, free fatty acids and glycerides was done by using different protocols.
In case of Labeo rohita, when exposed to textile industrial effluents ovaries showed the remarkable decline in the amount of lipid contents as the total lipids were measured 48.90 mg/g in control group while 32.66, 28.69 and 25.63 mg/g in sub-lethal concentrations T1, T2 and T3 respectively. Cholesterol 2.812 mg/g in Control group, 2.398, 1.960 and 1.712 mg/g in T1, T2 and T3 respectively. The mean of Phospholipids was 1.208 mg/g in control and 0.982, 0.732 and 0.566 in T1, T2 and T3 respectively. 4.196 mg/g Free Fatty Acids was in Control and 4.082, 3.758 and 3.322 mg/g in T1, T2 and T3 respectively. Similarly, the Glycerides were 40.69 mg/g in control group but 25.20, 22.24 and 20.03 in T1, T2 and T3, respectively.
Whereas in exposure to sewage effluent, the ovaries of Labeo rohita also showed the decreased amount of contents of lipids as follows:Total lipids was 48.90 mg/g for control group and 38.35, 36.32 and 32.77 mg/g in S1, S2 and S3 respectively. The mean of Cholesterol was 2.812 mg/g in Control group, 2.590, 2.224 and 2.196 mg/g in S1, S2 and S3 respectively. The mean of Phospholipids was 1.208 mg/g in control group and 1.164, 1.058 and 0.846 were in S1, S2 and S3 respectively, 4.196 mg/g of Free Fatty Acids were in Control group and 4.120, 3.900 and 3.478 mg/g in S1, S2 and S3 respectively. Similarly, the Glycerides were 40.69 mg/g in control group but 30.39, 29.12 and 26.25 mg/g in S1, S2 and S3 separately.