MICROBIAL POPULATION DIVERSITY IN PACIFIC ABALONE Haliotis discus hannai OF DIFFERENT GROWTH RATES

Microbial community in the intestine of marine organisms might reflect their physiological status. To study the relationship between an internal microbial population and the grow rate, hence the productivity, microbial populations in the intestine of Pacific abalone Haliotis discus hannai of different sizes were analyzed by using a culture-independent Illumina Mi-Seq analysis. Here we report the differences in the diversity of microbial populations depending on the sizes of the Pacific abalone.

Pacific abalones of 750 days post fertilization cultured in net cage collected were divided two, small and large, size groups. Intestine of each abalone were subject to DNA purification and its quality was assessed by gel electrophoresis. Quality of metagenomic DNAs extracted were tested by using PCR with 27F and 518R primers specific to the 16S rRNA gene.

SUMMARY

Microbial composition in intestine of Pacific abalone was identified using Illumina MiSeq.

Different (small and large) sizes of Pacific abalones were subjected to microbiome analysis. Microbial composition in each group was compared by species proportion and alpha-diversity indices. Small size abalone showed a more diverse microbial diversity than large abalone.